Polysaccharide derived from pomelo seed coat ameliorates APAP‐induced liver injury in hybrid grouper ( <i>Epinephelus lanceolatus</i> ♂ × <i>Epinephelus fuscoguttatus</i> ♀)

Ever-increasingly Citrus wastes have been generating during the industrial processing, which is troublesome to dispose them for considerations of economic feasibility and environmental protection. Meanwhile, liver disease, causes damage, one major threats further development aquaculture, especially in marine fish. The present study explored hepatoprotective effect PSCP, a polysaccharide extracted from pomelo seed coat, primary hepatocytes hybrid grouper (&lt;i&gt;Epinephelus fuscoguttatus&lt;/i&gt;♂ × &lt;i&gt;Epinephelus lanceolatus&lt;/i&gt;♀). PSCP displayed considerably scavenging free radicals strong inhibitive erythrocyte hemolysis. In vitro, we added (0, 100, 200 400 μg/mL) before incubation with Acetaminophen (APAP) (12 mM). CKK-8 experiment administration (100 substantially mitigated reduction hepatocyte viability inflicted by APAP. Moreover, found that effectively alleviated APAP-induced oxidative stress, as shown significantly reduced level reactive oxygen species elevated enzymatic activities superoxide dismutase, catalase, glutathione peroxidase, indicated its anti-liver injury (P&lt;0.05). more direct signs associating hepatoprotection are reflected results hematoxylin eosin staining, evident morphological recovery following addition PSCP. brief, these findings showed therapeutic potential on fish injury, not only provided now prospect treating impairment aquatic animals, but also improve utilization fruitlets.